Version History

1.x Series

1.0.1

Performance

  • Optimized vcf2fasta Pipeline: Substantially reduced execution time for variant-to-sequence conversion, enabling more efficient processing of large-scale VCF datasets.

  • Polyploid Genome Support: Extended compatibility to handle polyploid genomes, enabling analysis for a wider range of organisms.

User Interface

  • Empty VCF Warning: Users now receive a clear warning when uploading empty VCF files, preventing confusion and wasted processing time.

  • Configurable Result Display: Expanded rows-per-page options allow users to tailor the display density to their workflow and dataset size.

New Features

  • Chromosome-level Summary Table: New summary table displays total off-target sites per chromosome with allele-specific counts for each gRNA.

  • Advanced Result Filtering: Filter results by gRNA sequence, mismatch count, and GC content for more targeted analysis.

1.0.0

  • Initial public release of Variant-aware Cas-OFFinder — a variant-aware off-target prediction tool that incorporates individual genetic variants (via VCF files) alongside reference genomes.

  • Support for 40+ PAM types.

  • Unlimited mismatch tolerance for comprehensive off-target site detection.